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rabbit anti human collagen iv polyclonal antibody pab  (OriGene)


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    OriGene rabbit anti human collagen iv polyclonal antibody pab
    Rabbit Anti Human Collagen Iv Polyclonal Antibody Pab, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+collagen+iv+polyclonal+antibody+pab/Collagen+IV+(COL4A1)+Rabbit+Polyclonal+Antibody/pm25408555-31-25-33
    Average 90 stars, based on 6 article reviews
    rabbit anti human collagen iv polyclonal antibody pab - by Bioz Stars, 2026-11
    90/100 stars

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    Incubation:

    Article Title: AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival.
    Article Snippet: .. All rights reserved min) and incubated at room temperature (1 h) in 1% w/v BSA and 2% v/v FCS diluted in PBS before immunostaining with rabbit anti-human collagen IV polyclonal antibody (pAb) (#R1041, Acris Antibodies) and rabbit anti-human laminin pAb (#sc-5583, Santa Cruz), Samples were fixed and quenched (see above), washed with PBS (2 x 5 min), mounted in Vectashield® and analysed by confocal microscopy (Zeiss Confocal Axiovert 200M operated by LSM510 META software). .. For rheometry, 8 mm diameter x 1 mm thick rBM gels were analysed at 21°C with an ARG2 controlled strain rotational rheometer (TA Instruments) using 8 mm parallel plate serrated geometry, 1-3% strain and fixed frequency oscillation of 1 Hz.

    Immunostaining:

    Article Title: AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival.
    Article Snippet: .. All rights reserved min) and incubated at room temperature (1 h) in 1% w/v BSA and 2% v/v FCS diluted in PBS before immunostaining with rabbit anti-human collagen IV polyclonal antibody (pAb) (#R1041, Acris Antibodies) and rabbit anti-human laminin pAb (#sc-5583, Santa Cruz), Samples were fixed and quenched (see above), washed with PBS (2 x 5 min), mounted in Vectashield® and analysed by confocal microscopy (Zeiss Confocal Axiovert 200M operated by LSM510 META software). .. For rheometry, 8 mm diameter x 1 mm thick rBM gels were analysed at 21°C with an ARG2 controlled strain rotational rheometer (TA Instruments) using 8 mm parallel plate serrated geometry, 1-3% strain and fixed frequency oscillation of 1 Hz.

    Confocal Microscopy:

    Article Title: AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival.
    Article Snippet: .. All rights reserved min) and incubated at room temperature (1 h) in 1% w/v BSA and 2% v/v FCS diluted in PBS before immunostaining with rabbit anti-human collagen IV polyclonal antibody (pAb) (#R1041, Acris Antibodies) and rabbit anti-human laminin pAb (#sc-5583, Santa Cruz), Samples were fixed and quenched (see above), washed with PBS (2 x 5 min), mounted in Vectashield® and analysed by confocal microscopy (Zeiss Confocal Axiovert 200M operated by LSM510 META software). .. For rheometry, 8 mm diameter x 1 mm thick rBM gels were analysed at 21°C with an ARG2 controlled strain rotational rheometer (TA Instruments) using 8 mm parallel plate serrated geometry, 1-3% strain and fixed frequency oscillation of 1 Hz.

    Software:

    Article Title: AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival.
    Article Snippet: .. All rights reserved min) and incubated at room temperature (1 h) in 1% w/v BSA and 2% v/v FCS diluted in PBS before immunostaining with rabbit anti-human collagen IV polyclonal antibody (pAb) (#R1041, Acris Antibodies) and rabbit anti-human laminin pAb (#sc-5583, Santa Cruz), Samples were fixed and quenched (see above), washed with PBS (2 x 5 min), mounted in Vectashield® and analysed by confocal microscopy (Zeiss Confocal Axiovert 200M operated by LSM510 META software). .. For rheometry, 8 mm diameter x 1 mm thick rBM gels were analysed at 21°C with an ARG2 controlled strain rotational rheometer (TA Instruments) using 8 mm parallel plate serrated geometry, 1-3% strain and fixed frequency oscillation of 1 Hz.



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    Rabbit Anti Human Collagen Iv Polyclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti human collagen iv polyclonal antibody pab  (OriGene)
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    OriGene rabbit anti human collagen iv polyclonal antibody pab
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    https://www.bioz.com/product/rabbit+anti+human+collagen+iv+polyclonal+antibody+pab/Collagen+IV+(COL4A1)+Rabbit+Polyclonal+Antibody/pm25408555-31-25-33
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    Figure 4. Characterization of the target antigen by Western blot using anti-IgA ( chain specific) or anti-IgG ( chain specific) conjugates on various substrates. A, Dermal protein extracts. The patient’s IgA labeled a 185- to 190-kDa molecule (lane 1) that comigrated with the 5(IV) collagen chain as labeled by a specific antibody (lane 3); type VII collagen was labeled by serum from a patient with EBA (lane 2); none of the proteins in the extract were labeled by serum from a healthy individual (lane 4). B, Purified laminin-5. The patient’s IgA serum (lane 1) did not label the 165- to 200-kDa 3 laminin identified by a rabbit <t>polyclonal</t> antibody (lane 2). C, Recombinant 5(IV) noncollagenous (NC1) domain. The patient’s serum IgA labeled the 5(IV) NC1 domain at 1:100 dilution (lane 1). The 25-kDa recombinant 5(IV) NC1 domain that is bound to a Flag sequence was labeled by an anti-Flag antibody (lane 3) but not by a normal human serum (lane 2). Proteins were separated by SDS-PAGE using a 4% acrylamide gel for dermal lysates, gradient 4% to 20% for purified laminin-5, and 10% for recombinant proteins. The patient’s serum is indicated by an arrow.
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    Figure 4. Characterization of the target antigen by Western blot using anti-IgA ( chain specific) or anti-IgG ( chain specific) conjugates on various substrates. A, Dermal protein extracts. The patient’s IgA labeled a 185- to 190-kDa molecule (lane 1) that comigrated with the 5(IV) collagen chain as labeled by a specific antibody (lane 3); type VII collagen was labeled by serum from a patient with EBA (lane 2); none of the proteins in the extract were labeled by serum from a healthy individual (lane 4). B, Purified laminin-5. The patient’s IgA serum (lane 1) did not label the 165- to 200-kDa 3 laminin identified by a rabbit polyclonal antibody (lane 2). C, Recombinant 5(IV) noncollagenous (NC1) domain. The patient’s serum IgA labeled the 5(IV) NC1 domain at 1:100 dilution (lane 1). The 25-kDa recombinant 5(IV) NC1 domain that is bound to a Flag sequence was labeled by an anti-Flag antibody (lane 3) but not by a normal human serum (lane 2). Proteins were separated by SDS-PAGE using a 4% acrylamide gel for dermal lysates, gradient 4% to 20% for purified laminin-5, and 10% for recombinant proteins. The patient’s serum is indicated by an arrow.

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Crescentic glomerulonephritis and subepidermal blisters with autoantibodies to alpha5 and alpha6 chains of type IV collagen.

    doi: 10.1097/01.lab.0000067497.86646.4d

    Figure Lengend Snippet: Figure 4. Characterization of the target antigen by Western blot using anti-IgA ( chain specific) or anti-IgG ( chain specific) conjugates on various substrates. A, Dermal protein extracts. The patient’s IgA labeled a 185- to 190-kDa molecule (lane 1) that comigrated with the 5(IV) collagen chain as labeled by a specific antibody (lane 3); type VII collagen was labeled by serum from a patient with EBA (lane 2); none of the proteins in the extract were labeled by serum from a healthy individual (lane 4). B, Purified laminin-5. The patient’s IgA serum (lane 1) did not label the 165- to 200-kDa 3 laminin identified by a rabbit polyclonal antibody (lane 2). C, Recombinant 5(IV) noncollagenous (NC1) domain. The patient’s serum IgA labeled the 5(IV) NC1 domain at 1:100 dilution (lane 1). The 25-kDa recombinant 5(IV) NC1 domain that is bound to a Flag sequence was labeled by an anti-Flag antibody (lane 3) but not by a normal human serum (lane 2). Proteins were separated by SDS-PAGE using a 4% acrylamide gel for dermal lysates, gradient 4% to 20% for purified laminin-5, and 10% for recombinant proteins. The patient’s serum is indicated by an arrow.

    Article Snippet: The monoclonal and polyclonal antibodies used in this study were as follows: (i) a rabbit polyclonal antibody to type IV collagen purified from human placenta (Institut Pasteur de Lyon, Lyon, France); (ii) an mAb to either the 1(IV) or 3(IV) chain of type IV collagen (Wieslab, Lund, Sweden); (iii) polyclonal antibodies specific for the 5(IV) and 6(IV) chains of type IV collagen raised in mice in our laboratory (Dr. Ghohestani); (iv) a mouse mAb to type IV collagen (Dako SA, Trappes, France); (v) a mouse mAb to type VII collagen (Serotec, Oxford, England); (vi) a human mAb to the 230-kDa BP antigen (BP230) (Peyron et al, 1994); (vii) a rabbit polyclonal antibody to the immunodominant epitopes of the 180-kDa (BP180) ectodomain (R67) (Ghohestani et al, 1988); and (viii) a rabbit antibody to laminin-5 produced in our laboratory (Dr. Ghohestani).

    Techniques: Western Blot, Labeling, Purification, Recombinant, Sequencing, SDS Page, Acrylamide Gel Assay